PGLO Lab

 

pGLO Observations , Data Recording & Analysis

1.

Obtain your team plates.  Observe your set of  “+pGLO” plates under room light and with UV light.  Record numbers of colonies and color of colonies. Fill in the table below. Plate Number of Colonies Color of colonies under room light Color of colonies under   UV light - pGLO LB lawn yellow yellowish white - pGLO LB/amp 0 NA NA + pGLO LB/amp 62 yellow white + pGLO LB/amp/ara 350 yelow green

2.	What two new traits do your transformed bacteria have?
	The resistance to ampicillin and the GFP gene were the new traits our transformed bacteria had.
3.	Estimate how many bacteria were in the 100 uL of bacteria that you spread on each plate. Explain your logic.
	Each colony started with 1 bacteria. There would have been between 62 and millions of bacteria first. This is because 62 was the least number of colonies and it could have gone up to millions.
4.	What is the role of arabinose in the plates?
	The role of arabinose is to make the bacteria glow green by letting the RNA Polymerase through to the gene.
5.	List and briefly explain three current uses for GFP (green fluorescent protein) in research or applied science.
	The GFP is used because of its ability to generate a glowing color. GFP is used as a active indicator for protease action because then the scientists would know that it is working. Another reason why GFP is used is because it can glow inside an organism so the scientists could see what goes on inside the organisms cell. The third use is GFP is used for Biosensors, which allows you to analyze different conditions, like pH levels.
6.	Give an example of another application of genetic engineering.

In medicine, genetic engineering has been used to mass produce human growth hormones, insulin, monoclonal antibodies, vaccines and many more.